Mouse MYH4 (Myosin Heavy Chain 4, Skeletal Muscle) ELISA Kit

Mouse MYH4 (Myosin Heavy Chain 4, Skeletal Muscle) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MYH4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYH4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MYH4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MYH4 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: MYH2B; Myosin heavy chain 2b; Myosin heavy chain, skeletal muscle, fetal
• UniProt: Q5SX39
• Field of Research: Metabolic pathway.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 1000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 15.63-1000 pg/mL
• Sensitivity: 6.4 pg/mL