Rat CRHR2 (Corticotropin Releasing Hormone Receptor 2) ELISA Kit
CAT:
855-ELK6483-01
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Rat CRHR2 (Corticotropin Releasing Hormone Receptor 2) ELISA Kit
- Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CRHR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRHR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CRHR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CRHR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 7732-18-5
- Gene Aliases: CRFR2; CRF2; Corticotropin-releasing factor receptor 2
- UniProt: P47866
- Field of Research: Endocrinology.
- Assay Principle: Sandwich ELISA
- Assay Performance Time: 3.5h
- Standard: 20 ng/mL
- Sample Type: Tissue homogenates and other biological fluids.
- Detection Range: 0.32-20 ng/mL
- Sensitivity: 0.132 ng/mL