Rat PINP (Procollagen I N-Terminal Propeptide) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PⅠNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PⅠNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PⅠNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PⅠNP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide

• Gene Aliases:

  P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide

• Reactivity:

  Rat

• Field of Research:

  Metabolic pathway, Hepatology, Bone metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PINP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  200 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  3.13 - 200 ng/mL

• Sensitivity:

  1.18 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5