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Mouse SRGN (Serglycin) ELISA Kit

CAT: 0855-ELK7758-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7758-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SRGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SRGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SRGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SRGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PRG; PPG; PRG1; proteoglycan 1, secretory granule
UniProt
P13609
Reactivity
Mouse
Field of Research
Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SRGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SRGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SRGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SRGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
3000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
46.88-3000 pg/mL
Sensitivity
19.73 pg/mL

UniProtKB · P13609

Serglycin

SRGN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P13609
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Srgn
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q3TZD4, Q8C2U2
Protein keywords

Biological process

ApoptosisBiomineralization

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinProteoglycan

Cellular component

Golgi apparatusLysosomeSecreted

Domain

RepeatSignal