5-HT (5-Hydroxytryptamine) ELISA Kit

5-HT (5-Hydroxytryptamine) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: 5HT; Serotonin; N-Methyl-Gamma
• Field of Research: Signal transduction; Infection immunity; Neuro science; Gastroenterology.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 900 ng/mL
• Sample Type: Serum, plasma, tissue homogenates
• Detection Range: 14.07-900 ng/mL
• Sensitivity: 3.94 ng/mL