bHB (Beta-Hydroxybutyric Acid) ELISA Kit

bHB (Beta-Hydroxybutyric Acid) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bHB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of bHB in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
• Field of Research: Signal transduction; Metabolic pathway; Endocrinology.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 100 µg/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.57-100 µg/mL
• Sensitivity: 0.46 µg/mL