1,5-AG (1,5-Anhydroglucitol) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol

• Gene Aliases:

  LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol

• Reactivity:

  General

• Field of Research:

  Enzyme & Kinase, Metabolic pathway, Endocrinology, Nutrition metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  150 µg/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  2.35 - 150 µg/mL

• Sensitivity:

  0.6 µg/mL

• Species:

  General

• CAS Number:

  7732-18-5