17-OHP (17-Hydroxyprogesterone) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 17-OHP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-OHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone

• Reactivity:

  General

• Field of Research:

  Endocrinology; Reproductive science; Hormone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 17-OHP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-OHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2h

• Standard:

  20000 pg/mL

• Sample Type:

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  312.5-20000 pg/mL

• Sensitivity:

  87.6 pg/mL