8-epi-PGF2a (8-Epi Prostaglandin F2 Alpha) ELISA Kit

8-epi-PGF2a (8-Epi Prostaglandin F2 Alpha) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-epi-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: 8-EpiPGF2-α; 8epi-PGF2a
• Field of Research: Endocrinology; Pulmonology; Hepatology; Hormone metabolism.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 2000 pg/mL
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 31.25-2000 pg/mL
• Sensitivity: 9.23 pg/mL