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QA (Quinolinic Acid) ELISA Kit

CAT: 0855-ELK8141-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8141-01Size:48 Tests
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with QA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to QA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of QA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
QUIN; Pyridine-2,3-Dicarboxylic Acid
Reactivity
General
Field of Research
Enzyme & Kinase
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with QA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to QA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of QA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.54 ng/mL

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