Rat NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP (1-32) ; BNP (5-29) ; BNP-32; Gamma-brain natriuretic peptide; nppb

• Gene Aliases:

  NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb

• Reactivity:

  Rat

• Field of Research:

  Endocrinology, Cardiovascular biology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.057 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5, 9000-83-3