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NTM (Natamycin) ELISA Kit

CAT: 0855-ELK8241-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8241-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NTM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NTM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NTM in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Pimaricin; Natacyn
Reactivity
General
Field of Research
Infectious Diseases
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NTM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NTM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NTM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
300 ng/mL
Sample Type
Biological agents
Detection Range
4.69-300 ng/mL
Sensitivity
1.38 ng/mL

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