PIP3 (Phosphatidylinositol Trisphosphate) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  PtdIns (3,4,5) P3

• Gene Aliases:

  PtdIns(3,4,5)P3

• Reactivity:

  General

• Field of Research:

  Signal transduction

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  500 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  7.82 - 500 ng/mL

• Sensitivity:

  2.84 ng/mL

• Species:

  General

• CAS Number:

  7732-18-5