Products for Research Use Only
RegisterLogin

CH (Cholesterol) ELISA Kit

CAT: 0855-ELK8282-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK8282-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CH in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CH
Reactivity
General
Field of Research
Metabolic pathway; Hepatology; Gastroenterology; Nutrition metabolism; Hormone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
500 μg/mL
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 μg/mL
Sensitivity
2.41 μg/mL

Related Products

CatalogName
ELK8420-01Human TC (Total cholesterol) ELISA Kit
ELK11842-01Hamster TC (Total cholesterol) ELISA Kit
ELK8796-01Goat TC (Total cholesterol) ELISA Kit
ELK11735-01Fish TC (Total cholesterol) ELISA Kit
ELK9369-01Mouse TC (Total cholesterol) ELISA Kit
ELK8897-01Rat TC (Total cholesterol) ELISA Kit
ELK6122-01Rat LCAT (Lecithin Cholesterol Acyltransferase) ELISA Kit
ELK6471-01Mouse LCAT (Lecithin Cholesterol Acyltransferase) ELISA Kit
ELK3186-01Human LCAT (Lecithin Cholesterol Acyltransferase) ELISA Kit
ELK3800-01Human CH25H (Cholesterol-25-Hydroxylase) ELISA Kit

Alternative Products

CatalogName
abx258807-01Cholesterol ELISA Kit
E1701GeELISA Kit For Cholesterol
GTR10394336Human Cholesterol ELISA ELISA
GTR10437147Canine Cholesterol ELISA ELISA
GTR10417908Chicken Cholesterol ELISA ELISA
GTR10388487Porcine Cholesterol ELISA ELISA
GTR10374983Mouse Cholesterol ELISA ELISA
GTR10440883Bovine Cholesterol ELISA ELISA
GTR10437067Rabbit Cholesterol ELISA ELISA
GTR10475235Goat Cholesterol ELISA ELISA

Popular Products