DHEA-S (Dehydroepiandrosterone sulfate) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate

• Gene Aliases:

  Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate

• Reactivity:

  General

• Field of Research:

  Endocrinology, Reproductive science, Hormone metabolism, Rheumatology

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  400 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  6.25 - 400 ng/mL

• Sensitivity:

  4.44 ng/mL

• Species:

  General

• CAS Number:

  7732-18-5