Pig EGFR (Epidermal Growth Factor Receptor) ELISA Kit

Pig EGFR (Epidermal Growth Factor Receptor) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig EGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
• Field of Research: CD & Adhesion molecule; Tumor immunity.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 200 ng/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 3.13-200 ng/mL
• Sensitivity: 1.25 ng/mL