Avian 5-HT (5-Hydroxytryptamine) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  5HT; Serotonin; N-Methyl-Gamma

• Reactivity:

  Avian

• Field of Research:

  Signal transduction; Infection immunity; Neuro science; Gastroenterology

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2h

• Standard:

  900 ng/mL

• Sample Type:

  Serum and plasma

• Detection Range:

  14.07-900 ng/mL

• Sensitivity:

  4 ng/mL