Rat TBARS (Thiobarbituric Acid Reactive Substance) ELISA Kit
CAT:
855-ELK8905-02
Size:
96 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Rat TBARS (Thiobarbituric Acid Reactive Substance) ELISA Kit
- Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TBARS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 7732-18-5
- Gene Aliases: TBARS; Thiobarbituric Acid Reactive Substance
- Field of Research: Metabolic pathway; Hepatology; Hormone metabolism.
- Assay Principle: Competitive Inhibition ELISA
- Assay Performance Time: 2.5h
- Standard: 400 nmol/mL
- Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Detection Range: 6.25-400 nmol/mL
- Sensitivity: 3.6 nmol/mL