Anti-Chromogranin A(CGA414 + CGA/777 + CGA/798), CF594 conjugate

• Description:

  Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; Vasostatin I or II

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CHGA

• Gene ID:

  1113

• NCBI Gene ID:

  150793

• UniProt:

  P10645

• Cellular Locus:

  Vesicular

• Host:

  Mouse

• Species Reactivity:

  Human, Monkey, Mouse, Pig, Rat

• Immunogen:

  Recombinant human chromogranin A protein

• Target Antigen:

  CHGA | Chromogranin A

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  CGA414 CGA/777 CGA/798

• Conjugation:

  CF594

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Endocrinology

• Positive Control:

  PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  68-75 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4