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Mouse FETUB (Fetuin B) ELISA Kit

CAT: 0855-ELK9077-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK9077-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FETUB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FETUB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FETUB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FETUB in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
FETU-B; 16G2; Gugu; IRL685; Fetuin-like protein IRL685
UniProt
Q9QXC1
Reactivity
Mouse
Field of Research
Metabolic pathway; Infection immunity; Endocrinology; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FETUB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FETUB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FETUB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FETUB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
31.25-2000 ng/mL
Sensitivity
13.5 ng/mL

UniProtKB · Q9QXC1

Fetuin-B

FETUB_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9QXC1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Fetub
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

PTM

Disulfide bondGlycoproteinPhosphoprotein

Biological process

Fertilization

Molecular function

Metalloenzyme inhibitorMetalloprotease inhibitorProtease inhibitor

Domain

RepeatSignal

Cellular component

Secreted

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