Zebrafish GnRH (Gonadotropin Releasing Hormone) ELISA Kit

Zebrafish GnRH (Gonadotropin Releasing Hormone) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
• Field of Research: Endocrinology; Reproductive science; Neuro science; Genetic science; Hormone metabolism.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 16 ng/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.25-16 ng/mL
• Sensitivity: 0.1 ng/mL