Mouse PDZK1IP1 (PDZK1-interacting protein 1) ELISA Kit

Mouse PDZK1IP1 (PDZK1-interacting protein 1) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDZK1IP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDZK1IP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDZK1IP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDZK1IP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: MAP17; 17 kDa membrane associated protein; DD96; Epithelial protein up regulated in carcinoma membrane associated protein 17; Membrane associated protein 17; PDZK1-interacting protein 1; Pdzk1ip1; RP1-18D14.5; SPAP;
• Field of Research: Cancer.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 2000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 31.25-2000 pg/mL
• Sensitivity: 13.3 pg/mL