Human PC (Protein Carbonyl) ELISA Kit

Human PC (Protein Carbonyl) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PC in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Protein Carbonyl
• Field of Research: Metabolic pathway; Hepatology; Hormone metabolism.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 nmol/mL
• Sample Type: Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate
• Detection Range: 0.32-20 nmol/mL
• Sensitivity: 0.117 nmol/mL