Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit

Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: TC2; Transcobalamin II
• Field of Research: Neuro science.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 5000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 29 pg/mL