Zebrafish 5-HT (5-Hydroxytryptamine) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  5HT; Serotonin; N-Methyl-Gamma

• Gene Aliases:

  5HT; Serotonin; N-Methyl-Gamma

• Reactivity:

  Zebrafish

• Field of Research:

  Signal transduction, Infection immunity, Neuro science, Gastroenterology

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  900 ng/mL

• Sample Type:

  Serum, plasma

• Detection Range:

  14.07 - 900 ng/mL

• Sensitivity:

  3.85 ng/mL

• Species:

  Zebrafish

• CAS Number:

  7732-18-5