Cattle LDH (Lactate Dehydrogenase) ELISA Kit

Cattle LDH (Lactate Dehydrogenase) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LDH in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Lactate Dehydrogenase
• Field of Research: Enzyme & Kinase; Metabolic pathway; Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 50 ng/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.79-50 ng/mL
• Sensitivity: 0.34 ng/mL