Rat SEMA3F (Semaphorin 3F) ELISA Kit

Rat SEMA3F (Semaphorin 3F) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SEMA3F. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SEMA3F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SEMA3F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SEMA3F in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: SEMA-IV; SEMA4; SEMAK; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F
• Field of Research: CD & Adhesion molecule.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 1000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 15.63-1000 pg/mL
• Sensitivity: 5.9 pg/mL