Anti-CDw17(HuLy-m13)

• Description:

  CDw17 / CD17 is an intermediate glycosphingolipid from the metabolism of higher gangliosides that localizes to sphingolipid-sterol rafts. CD17 is detectable in monocytes, granulocytes, basophils, platelets, a subset of peripheral B cells (CD19 ) and tonsil dendritic cells. It is rapidly down regulated on activated granulocytes and is upregulated on IL-2 activated T lymphocytes. CD17 binds to bacteria and may function in phagocytosis. VEGF-treated endothelial cells can produce CD17, which can then mediate signaling toward PECAM-1 expression and angiogenesis. Tumor necrosis factor alpha (TNF-alpha)-induced astrogliosis (astrocyte proliferation and glial fibrillary acidic protein upregulation) in response to neuro-inflammation (i.e. spinal cord injury) causes an increase in intracellular levels of CD17. Aberrant levels of glycosphingolipids are a feature of cancer cells and may influence integrin clustering and internalization._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_ This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

• Synonyms:

  Lactosylceramide; LacCer; ?-D-galactosyl-(1-4)-?-D-glucosyl-(1-1')-ceramide; CDw17

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene ID:

  Not Known

• NCBI Gene ID:

  Not Known

• UniProt:

  Not Known

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  β-2 Microglobulin associated proteins from a detergent lysate of human PBL

• Target Antigen:

  CDw17

• Clonality:

  Monoclonal

• Isotype:

  IgM

• Clone:

  HuLy-m13

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Functional studies (published) | IP (published) | WB (published)

• Validated Applications:

  IP, WB

• Field of Research:

  Cancer, Immunology, Inflammation

• Positive Control:

  Human PBL. Tonsil

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  Not Known

• Additionnal Information:

  Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Blood 2002 100(4): 1454-1464. (functional studies; IP; WB)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4