CD86 (BU63), 0.2mg/mL

• Description:

  Recognizes a protein of 70 kDa, which is identified as CD86. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4, a cancer immunotherapy target. It is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Activation B7-2 antigen|B lymphocyte activation antigen B72|CD28 antigen ligand 2|CD28LG2|CLS1|CTLA-4 counter-receptor B7.2|Early T-cell co-stimulatory molecule 1|ETC-1|FUN-1|LAB72|Ly-58|MB7-2|TS/A-2

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD86

• Gene ID:

  942

• NCBI Gene ID:

  171182

• UniProt:

  P42081

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  ARH-77 (B-lymphoblastoid cell line)

• Target Antigen:

  CD86

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  BU63

• Conjugation:

  Purified, with BSA

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  Flow (verified) |Flow, surface (published) |Functional studies (published) |IF (verified) |IHC, FFPE (verified)

• Validated Applications:

  FC, FC, IF, IHC, FFPE

• Field of Research:

  Cancer immunotherapy/immune checkpoint, Immunology

• Positive Control:

  293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  70 kDa

• Additionnal Information:

  Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Immunoprecipitation 0.5-1 ug/500 ug protein lysate|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (1998) 161: 6375-6382. (functional studies; Flow, surface)

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA