Anti-CD74(BU45)

• Description:

  Recognizes proteins of 33, 35 and 41 kDa, which are identified as various isoforms of CD74. Its epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma ( ).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CLIP; DHLAG; Gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLADR-gamma (HLADG); Ia antigen-associated invariant chain; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA-DR gamma chain

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD74

• Gene ID:

  972

• NCBI Gene ID:

  436568

• UniProt:

  P044233

• Cellular Locus:

  Plasma membrane|Perinuclear

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  B lymphoblastoid cell line HFB1

• Target Antigen:

  CD74

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  BU45

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow, surface (published) | IP (published)

• Validated Applications:

  FC, IP

• Field of Research:

  Immunology

• Positive Control:

  Daudi Cells. Tonsil or lymph node.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  33-41 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1995) 84: 177-182. (IP, Flow)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4