Anti-CD71(DF1513), CF640R conjugate

• Description:

  This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa (Workshop IV). This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TFRC

• Gene ID:

  7037

• NCBI Gene ID:

  529618

• UniProt:

  P02786

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  KG1 acute myeloid leukemia cell line

• Target Antigen:

  CD71 | Transferrin Receptor

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  DF1513

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified) | WB (verified)

• Validated Applications:

  FC, IF, WB

• Field of Research:

  Cancer

• Positive Control:

  CCRF-CEM, Jurkat or K-562. Human placenta or breast carcinoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  85-95 kDa (monomer); 190 kDa (dimer)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1.0 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4