Anti-CD7(T3-3A1), CF640R conjugate

• Description: Recognizes a protein of 40 kDa, identified as CD7, a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD27 ligand (CD27L); CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD7
• Gene ID: 924
• NCBI Gene ID: 186820
• UniProt: P09564
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human T cells
• Target Antigen: CD7
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: T3-3A1
• Conjugation: CF640R
• Disease: Tumor
• Source: Animal
• Applications: Flow, surface (published) | Functional studies (published) | IF (published) | ELISA (published) | IP (published)
• Validated Applications: FC, IF, ELISA, IP
• Field of Research: Immunology, Signal transduction
• Positive Control: Jurkat, HUT-78, Molt-4, CEM cells, or human peripheral blood lymphocytes. Lymph node and tonsil.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 40 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1979) 76: 5829-5833. PNAS USA (1980) 77(5): 2914-2918. (IP; functional studies) J Immunol Meth (1987) 96(2), 201-209. (cell ELISA) PNAS USA (1996) 93: 10348-10353. (cell depletion)>/li> Blood (1998) 91(8) 2760-2771. (Flow) J Cell Biol (1998) 142(5): 1245-1256. (IF; IP) Theranostics (2018) 8(21): 6070-6087. (mAb characterization; in vivo imaging)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years