Anti-CD6(C6/372 + 3F7B5), CF740 conjugate
CAT:
37-BNC741228-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-CD6(C6/372 + 3F7B5), CF740 conjugate
- Description: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: T12; TP120
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: CD6
- Gene ID: 923
- NCBI Gene ID: 744366
- UniProt: P30203
- Cellular Locus: Plasma membrane
- Host: Mouse
- Species Reactivity: Human
- Immunogen: Human recombinant CD6 protein (C6/372); Human rheumatoid synovial T cell line ST-1 (3F7B5)
- Target Antigen: CD6
- Clonality: Monoclonal
- Isotype: IgG
- Clone: C6/372 3F7B5
- Conjugation: CF740
- Source: Animal
- Applications: IF (verified) | IHC, FFPE (verified)
- Validated Applications: IF, IHC, FFPE
- Field of Research: Immunology
- Positive Control: CCRF-CEM, Jurkat cells, Tonsil
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% rBSA, 0.05% azide
- Molecular Weight: 90-130 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years