Anti-CD57 / B3GAT1(NK-1)

• Description:

  Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA._x000D_ _x000D_ This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

• Synonyms:

  3-Glucuronyltransferase 1; B3GAT1; Galactosylgalactosylxylosylprotein 3-beta-Glucuronosyltransferase 1; GLCATP; GlcUAT-P; Glucuronosyltransferase P; UDP GlcUA Glycoprotein beta 1, 3 Glucuronyltransferase

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  B3GAT1

• Gene ID:

  27087

• NCBI Gene ID:

  381050

• UniProt:

  Q9P2W7

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human peripheral blood mononuclear cells

• Target Antigen:

  B3GAT1 | CD57

• Clonality:

  Monoclonal

• Isotype:

  IgM κ

• Clone:

  NK-1

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow, surface (published) | IF (published) | IHC, FFPE (verified) | WB (published)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Cancer, Immunology

• Positive Control:

  Lymph node or Tonsil

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  ~110 kDa (Glycoprotein)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with rat; others not known|Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Asian J Androl (2010) 12(4): 548-555. (WB blot; Flow) Cancer Immunol Immunother (2011) 60:1683-1695. (Flow, surface) Cancer Sci (2016) 107: 846-852. (IHC, FFPE) Cell Stem Cell (2017) 20(6): 874-890.e7. (IF; Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4