Anti-CD55 (DAF)(F4-29D9)

• Description:

  Recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD55|CD55 Cromer blood group system|Complement decay-accelerating factor|CROM|DAF|Daf-GPI|Decay Accelerating Factor for Complement|Decay accelerating factor GPI-form|Decay accelerating factor soluble-form|GPI-DAF

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD55

• Gene ID:

  1604

• NCBI Gene ID:

  126517

• UniProt:

  P08174

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human umbilical vein endothelial cells (HUVEC)

• Target Antigen:

  CD55

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  F4-29D9

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified)

• Validated Applications:

  FC, IF

• Field of Research:

  Cell adhesion, Immunology

• Positive Control:

  Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes. Human lymph nodes and tonsils.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  70 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4