Recombinant Human Epigen

• Background:

  Epigen is a member of the epidermal growth factor family and is expressed by several tissues, including the testis, liver, heart and in certain tumor cells. It is strongly mitogenic for fibroblasts and epithelial cells, despite its relatively weak affinity for its main receptor, ErbB1. The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion. The gene of human Epigen encodes a 154 amino acid (a.a.) residue type I transmembrane precursor glycoprotein which contains a 22 a.a. signal peptide, an 88 a.a. extracellular domain, a 21 a.a. transmembrane domain and a 23 a.a. cytoplasmic domain. By alternative splicing of the gene, Epigen has several isoforms. The mature, shed form of human Epigen (54 104a.a.) shares 92 %, 94 % and 94 % a.a. sequence identity with mouse, rat and equine Epigen, respectively.

• Product Name Alternative:

  EPG, Epithelial Mitogen

• Gene ID:

  255324

• UniProt:

  Q6UW88

• Target:

  AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

• Purification:

  > 98 % by SDS-PAGE and HPLC analyses.

• Endotoxin:

  Less than 1 EU/ug of rHuEpigen as determined by LAL method.

• Bioactivity:

  3

• Storage Conditions:

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

• Calculated Molecular Weight:

  Approximately 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor.

• Formulation:

  Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

• Host or Source:

  Escherichia coli