Anti-CD48(5-4.8), CF640R conjugate

• Description:

  Reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  B cell membrane protein; B-cell activation marker; BCM1 surface antigen; BLAST1; Signaling lymphocytic activation molecule 2; SLAM family member 2 (SLAMF2); TCT.1

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD48

• Gene ID:

  962

• NCBI Gene ID:

  243564

• UniProt:

  P09326

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human peripheral blood lymphocytes

• Target Antigen:

  CD48

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  5-4.8

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | WB (published)

• Validated Applications:

  FC, WB

• Field of Research:

  Immunology

• Positive Control:

  Daudi, JY, Raji, Jurkat, and human lymphocytes. Human lymph node and tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  45 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Exp Med (1996) 184: 1769-1779. (functional studies)>/li> J Immunol (2001) 166:5567-5577. (Flow; functional studies; WB)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4