Anti-CD46(169-1-E4.3)

Anti-CD46(169-1-E4.3)

• Description: Recognizes a protein of 52 kDa-58 kDa, identified as CD46 (also known as membrane cofactor protein, MCP). Its epitope is located on the four immunogenic short consensus repeat (SCR) domains at N-terminus of the protein. CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: AHUS2; Complement membrane cofactor protein; MCP; Measles virus receptor; Membrane cofactor protein; MIC10; TLX; Trophoblast leucocyte common antigen; Trophoblast lymphocyte cross reactive antigen
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD46
• Gene ID: 4179
• NCBI Gene ID: 510402
• UniProt: P15529
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Baboon, Human
• Immunogen: Stimulated human leukocytes
• Target Antigen: CD46
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: 169-1-E4.3
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: Flow, surface (published) | IHC, FFPE (published) | Functional studies (published)
• Validated Applications: FC, IHC, FFPE, Functional assays
• Field of Research: Immunology
• Positive Control: K562, U937, HL60, HeLa and Jurkat cells, and human lymphocytes. Lymph node and tonsil.
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 52-58 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with horse, others not tested|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Anticancer Res (2006) 26: 4943-4948. (IHC, FFPE) Virology (2017) 502: 160-170. (functional studies) Virology (2010) 405(2), 474-482.
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years