Anti-CD45RA(111-1C5), CF647 conjugate
CAT:
37-BNC471038-100
Size:
100 µL
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-CD45RA(111-1C5), CF647 conjugate
- Description: Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO positive) from B cell lymphomas (CD45RA positive).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: PTPRC
- Gene ID: 5788
- NCBI Gene ID: 654514
- UniProt: P08575
- Cellular Locus: Plasma membrane
- Host: Mouse
- Species Reactivity: Human
- Immunogen: Stimulated human leukocytes
- Target Antigen: CD45RA
- Clonality: Monoclonal
- Isotype: IgG1 κ
- Clone: 111-1C5
- Conjugation: CF647
- Disease: Tumor
- Source: Animal
- Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
- Validated Applications: FC, IF, IHC, FFPE, WB
- Field of Research: Immunology, Signal transduction
- Positive Control: Daudi, Raji, IM9 and human lymphocytes. Human lymph nodes and tonsils.
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% BSA, 0.05% azide
- Molecular Weight: 205-220 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years