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CD3e (RIV9), 0.2mg/mL

CAT: 0037-BNUB0322-100Size: 1x 100 µLDry Ice: NoHazardous: No
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CAT#:0037-BNUB0322-100Size:1x 100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Reacts with five invariable CD3 chains designated CD3y, CD3d, CD3e, CD3f, and CD3n with molecular weight ranging from 16-28 kDa. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
CAS Number
9007-83-4
Synonyms
CD3 epsilon | CD3 TCR complex | CD3E | CD3e antigen epsilon polypeptide (TiT3 complex) | Leu-4 epsilon chain | T cell antigen receptor complex epsilon subunit of T3 | T-cell surface antigen T3 | T-cell surface glycoprotein CD3 epsilon chain | T3E | TCRE | TiT3 complex
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
CD3E
Gene ID
916
NCBI Gene ID
3003
UniProt
P07766
Swiss Prot
P07766
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Reactivity
CD3
Immunogen
Human peripheral lymphocytes
Target Antigen
CD3
Clonality
Monoclonal
Clone
RIV9
Type
Primary
Source
Animal
Origin
Animal
Applications
Functional studies (published) |IHC, frozen (expected) |Flow, surface (verified) |IF (verified) |IP (published)
Field of Research
Immunology
Positive Control
Jurkat cells. Tonsil and lymph node
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
20 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Transplant Proc (1989) 21: 1026-7. (functional studies) Eur J Immunol (1990) 20(4): 943-945. (immunoprecipitation) Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989. Immunology (1994) 83: 457-464. (immunofluorescence, frozen tissue) Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995.
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG3, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA
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