Anti-CD3e(CRIS-7), CF555 conjugate

Anti-CD3e(CRIS-7), CF555 conjugate

• Description: Recognizes the epsilon chain of CD3 (Workshop V; Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: CD 3E|CD3 epsilon|CD3 TCR complex|CD3E|CD3e antigen epsilon polypeptide (TiT3 complex)|T cell antigen receptor complex epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain|T3E|TCRE|TiT3 complex
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD3E
• Gene ID: 916
• NCBI Gene ID: 3003
• UniProt: P07766
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Monkey
• Immunogen: Stimulated human leukocytes
• Target Antigen: CD3e
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: CRIS-7
• Conjugation: CF555
• Disease: Tumor
• Source: Animal
• Applications: Functional studies (published) | Flow, surface (verified) | IF (verified)
• Validated Applications: FC, IF
• Field of Research: Immunology
• Positive Control: Jurkat cells. Tonsil and lymph node
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 20 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Induce T cell activation and proliferation|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. McMichael AJ et al. (eds) Leukocyte Typing III, Oxford University Press, Oxford, 1987 Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989. Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. J Immunol 1991, 146(4):108 (functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years