CD3e (CRIS-7), 0.2mg/mL

• Description :

  Recognizes the epsilon chain of CD3 (Workshop V; Code: CD03.09), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms :

  CD 3E|CD3 epsilon|CD3 TCR complex|CD3E|CD3e antigen epsilon polypeptide (TiT3 complex) |T cell antigen receptor complex epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain|T3E|TCRE|TiT3 complex

• UNSPSC :

  41116161

• UNSPSC Description :

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name :

  CD3E

• Gene ID :

  916

• NCBI Gene ID :

  3003

• UniProt :

  P07766

• Swiss Prot :

  P07766

• Host :

  Mouse

• Species Reactivity :

  Human, Monkey

• Reactivity :

  CD3e

• Immunogen :

  Stimulated human leukocytes

• Target Antigen :

  CD3e

• Clonality :

  Monoclonal

• Clone :

  CRIS-7

• Type :

  Primary

• Source :

  Animal

• Origin :

  Animal

• Applications :

  Functional studies (published) |Flow, surface (verified) |IF (verified)

• Field of Research :

  Immunology

• Positive Control :

  Jurkat cells. Tonsil and lymph node

• Stability :

  2 years

• Concentration :

  0.2 mg/mL

• Buffer :

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight :

  20 kDa

• References & Citations :

  Note: References for this clone sold by other suppliers may be listed for expected applications. McMichael AJ et al. (eds) Leukocyte Typing III, Oxford University Press, Oxford, 1987 Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989. Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. J Immunol 1991, 146(4):108 (functional studies)

• Shipping Conditions :

  RT (ND)

• Storage Conditions :

  Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life :

  Guaranteed for at least 24 months from date of receipt when stored as recommended

• Fragment :

  IgG2a, kappa

• Species :

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification :

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number :

  9007-83-4

• Conjugate :

  Purified, with BSA