Anti-Nucleolin(364-5 + NCL/902), CF740 conjugate

Anti-Nucleolin(364-5 + NCL/902), CF740 conjugate

• Description: Recognizes Nucleolin (NCL), the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11 kb. It has a predicted molecular weight of ~76 kDa with an apparent MW of 100-110 kDa, attributed to phosphorylation of its N-terminal domain. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: NCL; Nucl; Nucleolin; Protein C23
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: NCL
• Gene ID: 4691
• NCBI Gene ID: 79110
• UniProt: P19338
• Cellular Locus: Nucleoli
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
• Target Antigen: Nucleolin
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 364-5 NCL/902
• Conjugation: CF740
• Source: Animal
• Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, WB
• Field of Research: Organelle markers
• Positive Control: All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin's lymphoma
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 76 kDa (predicted), 100-110 kDa (apparent)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with mouse, rat or cow; others not known|Immunohistology (formalin)|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years