Anti-CD22(Myg13), CF640R conjugate

Anti-CD22(Myg13), CF640R conjugate

• Description: This antibody ecognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: B-cell receptor CD22|B-lymphocyte cell adhesion molecule (BL-CAM)|Lectin 2|Lyb8|Sialic acid-binding Ig-like lectin 2 (Siglec-2)|SIGLEC2|T-cell surface antigen Leu-14
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CD22
• Gene ID: 933
• NCBI Gene ID: 579691
• UniProt: P20273
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Human Raji cells
• Target Antigen: BL-CAM | CD22
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: Myg13
• Conjugation: CF640R
• Source: Animal
• Applications: Flow (verified) | Flow, surface (published) | Functional studies (published) | WB (published)
• Validated Applications: FC, FC, WB
• Field of Research: Immunology
• Positive Control: Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human tonsil and lymphocytes.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 130-140 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Glia (2004) 46: 369-379. (functional studies; WB) Exp Mol Pathol (2010) 88(2): 238-249. (Flow, surface) J Immunol (2011) 186: 1554-1563. (cell internalization)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years