Anti-CD171 / L1CAM (L1 Cell Adhesion Molecule)(UJ127), Biotin conjugate

• Description:

  Recognizes a cell surface protein of 220-240 kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  L1CAM

• Gene ID:

  3897

• NCBI Gene ID:

  52818

• UniProt:

  P32004

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Homogenous suspension of 16 week human fetal brain

• Target Antigen:

  CD171 | L1CAM

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  UJ127

• Conjugation:

  Biotin

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow, surface (published) | IHC, FFPE (published) | IP (published) | WB (published)

• Validated Applications:

  FC, IHC, FFPE, IP, WB

• Field of Research:

  Cancer, Cell adhesion, Developmental biology

• Positive Control:

  Neuroblastomas or Schwannomas

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  220-240 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Eur J Gynaecol Oncol (2008) 29(1):26-30. (IHC, FFPE; WB) Anticancer Res (2009) 29: 1195-1200. (IHC, FFPE) Mol Biol Cell (2010) 21: 1671-1685. (IHC, FFPE; Flow, IP)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4