Anti-CD16(HuNK2)

Anti-CD16(HuNK2)

• Description: Recognizes a protein of 50-65 kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This MAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; PSGL1; Selectin P ligand; SELPLG
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: FCGR3A
• Gene ID: 2214
• NCBI Gene ID: 372679
• UniProt: P08637
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human peripheral blood lymphocytes from a NK-leukemia patient.
• Target Antigen: CD16 | Fc gamma receptor III
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: HuNK2
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: Flow, surface (published)
• Validated Applications: FC,
• Field of Research: Immunology
• Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 50-80 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Functional Studies order Ab without BSA & azide)|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Blood (2001) 98(1):140-145. (Flow, surface) Blood (2002) 100(13): 4512-4520. (cell depletion)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years