CD147 (CB43), 0.2mg/mL

• Description:

  CD147 is a transmembrane glycoprotein of the immunoglobulin superfamily. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is involved in the regulation of matrix remodeling at the epidermal-dermal interface. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

• Synonyms:

  L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD4

• Gene ID:

  920

• NCBI Gene ID:

  631659

• UniProt:

  P01730

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Monkey

• Immunogen:

  Human peripheral lymphocytes

• Target Antigen:

  CD4

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  RIV7

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Type:

  Primary

• Source:

  Animal

• Origin:

  Animal

• Applications:

  Functional studies (published) |Flow, surface (verified) |IF (verified)

• Validated Applications:

  FC, IF

• Positive Control:

  Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.

• Stability:

  2 years

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  Functional studies: Order Ab without azide; Flow cytometry: 1-2 ug/million cells in 0.1 mL; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp W. et al. (eds) Leukocyte Typing IV, p314-330, Oxford University Press, Oxford, 1989. Br J Haematol (2004) 128: 73-81. (functional studies)

• Shipping Conditions:

  RT (ND)

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• Species:

  Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

• Classification:

  PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

• CAS Number:

  9007-83-4

• By Conjugate:

  Purified, with BSA