Anti-CD11c(HC1/1), CF640R conjugate

• Description:

  CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells. It induces cellular activation and helps trigger neutrophil respiratory burst. It is expressed in hairy cell leukemias, acute non-lymphocytic leukemias, and some B-cell chronic lymphocytic leukemias._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  CD11 antigen-like family member B|CD11b/CD18|CD49d|Cell surface glycoprotein MAC-1 subunit alpha|Complement Component Receptor 3 Alpha|CR3 Alpha Chain (CR3A)|Integrin alpha-M|Integrin beta 2 alpha subunit|ITGAM|Leukocyte adhesion receptor MO1; Ly-40|Mac-1a|MAC1|Mac1, alpha subunit|MAC1A|Macrophage antigen alpha polypeptide; MO1A|Neutrophil adherence receptor alpha M subunit

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ITGAX

• Gene ID:

  3687

• NCBI Gene ID:

  248472

• UniProt:

  P20702

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human monocytic U937 phorbol ester-treated cells

• Target Antigen:

  CD11c

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  HC1/1

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | Flow, surface (published) | Functional studies (published) | IP (published)

• Validated Applications:

  FC, FC, IP

• Field of Research:

  Cell adhesion, Immunology

• Positive Control:

  Human dendritic cells. Human lymph nodes and tonsils.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  145 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Hybridoma (1988) 7:2, 167-176. (IHC) J Exp Med (1991) 174 (6): 1313-1322. (Flow; receptor activation; IP)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years