Anti-CD11a(DF1524)

• Description:

  Recognizes a protein of 180 kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This MAb potently blocks LFA-1 dependent homotypic cell aggregation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; p180

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ITGAL

• Gene ID:

  3683

• NCBI Gene ID:

  174103

• UniProt:

  P20701

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human CD11a protein

• Target Antigen:

  CD11a

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  DF1524

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | IF (published)

• Validated Applications:

  FC, IF

• Field of Research:

  Immunology

• Positive Control:

  JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  180 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Neuropathol Exp Neurol (2000) 59(1): 18-28. (functional studies) FASEB J (2003) 17(11), 1-21. (Flow) Blood (2006) 107(8): 3212-3220. (IF)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4