Anti-CD11a(Cris-3), CF543 conjugate

Anti-CD11a(Cris-3), CF543 conjugate

• Description: Recognizes a protein of 180 kDa, identified as CD11a (Leucocyte Workshop II; Code N202). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; p180
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ITGAL
• Gene ID: 3683
• NCBI Gene ID: 174103
• UniProt: P20701
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Stimulated human leukocytes
• Target Antigen: CD11a
• Clonality: Monoclonal
• Isotype: IgG2b κ
• Clone: Cris-3
• Conjugation: CF543
• Source: Animal
• Applications: Flow (verified) | Functional studies (published)
• Validated Applications: FC, Functional assays
• Field of Research: Immunology
• Positive Control: JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 180 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp W. et al. Leukocyte Typing IV, Oxford Unioversity Press, Oxford, 1989. Reinherz EL et al. eds. Leukocyte Typing II, Oxford University Press, Oxford, 1985. J Exp Med (1990) 172: 335-345. (Flow, surface) Am J Physiol Cell Physiol (1998) 274(6): C1634-C1644. (receptor blockade).
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years